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l lg ythdf1 proteintech  (Proteintech)


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    Proteintech l lg ythdf1 proteintech
    L Lg Ythdf1 Proteintech, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 409 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ythdf1+proteintech+66745+1+ig/YTHDF1+Antibody/pm41520337-278-73-75
    Average 96 stars, based on 409 article reviews
    l lg ythdf1 proteintech - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Western Blot:

    Article Title: YTHDF2 regulates self non-coding RNA metabolism to control inflammation and tumorigenesis
    Article Snippet: Antibodies for IF staining Reagents or Resource Source Identifier Dilution Alexa Fluor® 488 Donkey Anti-Guinea Pig IgG (H+L) Jackson ImmunoResearch 706-545-148 1/100 Alexa Fluor® 488 Donkey Anti-Mouse IgG (H+L) Jackson ImmunoResearch 715-545-150 1/100 Alexa Fluor® 594 Donkey Anti-Rabbit IgG (H+L) Jackson ImmunoResearch 711-585-152 1/100 Pan-cytokeratin Origene BP5069 1/200 RAB7 Abcam Ab50533 1/100 YTHDF2 (IHC and IF) Proteintech #247441-1-AP 1/500 YTHDF2 (Confocal) Abcam ab246514 1/200 dsRNA (IF) SCIONS 10010200 1/150 Cytokeratin 14 (IF) abcam Ab7800 1/1000 Cytokeratin 10 (IF) abcam Ab76318 1/5000 COX-2 (IF) Santa Cruz sc-376861 1/50 Supplementary Table S5. .. Antibodies for immunoblotting Reagents or Resource Source Identifier Dilution ATG5 Cell Signaling Technology 2630S 1/1000 ATG7 Cell Signaling Technology 8558S 1/1000 beta-actin Santa Cruz SC-47778 1/10000 COX-2 Santa Cruz SC-19999 1/500 FLAG-Tag (DYKDDDDK) Cell Signaling Technology 8146S 1/500 FLAG-Tag (DYKDDDDK) Cell Signaling Technology 14793S 1/500 FLAG-Tag (DYKDDDDK) Proteintech 20543-1-AP 1/2000 GAPDH Santa Cruz SC-47724 1/10000 HA-Tag Cell Signaling Technology 3724S 1/2000 HA-Tag Sigma H3663 1/2000 IGF2BP1 (IMP1) Cell Signaling Technology 8482S 1/1000 IGF2BP2 (IMP2) Cell Signaling Technology 14672S 1/1000 IGF2BP3 (IMP3) Cell Signaling Technology 57145S 1/1000 LC3B Cell Signaling Technology 3868S 1/500 METTL16 Cell Signaling Technology 17676S 1/2000 p62 Sigma P0067 1/2000 p62 Progen Biotechnik GmbH GP62-C 1/10000 TLR3 Cell Signaling Technology 6961S 1/500 TLR3 Abcam ab62566 1/500 TLR3 Abcam ab13915 1/500 YTHDC1 Abcam ab122340 1/1000 YTHDC2 Abcam ab176846 1/1000 YTHDF1 Proteintech 66745-1-Ig 1/2000 YTHDF2 Abcam ab220163 1/5000 YTHDF2 Proteintech 247441-1-AP 1/5000 YTHDF2 Cell Signaling Technology 80014S 1/2000 YTHDF3 Cell Signaling Technology 24206S 1/500 PARP1 Santa Cruz sc-8007 1/500 RAB5 Abcam ab18211 1/500 RAB7 Abcam ab50533 1/1000 YTHDF2 pS39 Ab Generated by ABclonal 1/1000 CNOT1 Proteintech 66507-1-lg 1/500 Supplementary Table S6. .. Antibodies for flow cytometric analysis Antibodies Clone Source Identifier FITC anti-mouse CD103 Antibody 2 E7 Biolegend 121420 FITC anti-mouse CD3 Antibody 17A2 Biolegend 100203 FOXP3 Monoclonal Antibody, PE NRRF-30 Thermo fisher 12-4771-82 PerCP/Cy5.5 anti-mouse/human CD45R/B220 Antibody RA3-6B2 Biolegend 103236 PerCP/Cyanine5.5 anti-mouse/human CD11b Antibody 101228 Biolegend 101228 PE/Cy7 anti-mouse CD279 (PD-1) Antibody 29F.1A12 Biolegend 109110 PE/Cyanine7 anti-mouse/human CD44 Antibody IM7 Biolegend 103030 PE/Cyanine7 anti-mouse Ly-6C Antibody HK1.4 Biolegend 128018 APC anti-mouse CD11C Antibody N418 Biolegend 117310 APC anti-mouse CD62L Antibody MEL-14 Biolegend 104412 Brilliant Violet 421TM anti-mouse I-A/I-E Antibody M5/114.15.2 Biolegend 107632 Brilliant Violet 421TM anti-mouse NK-1.1 Antibody PK136 Biolegend 100451 Brilliant Violet 510TM anti-mouse Ly-6G Antibody 1A8 Biolegend 127633 Brilliant Violet 605TM anti-mouse CD4 Antibody GK1.5 Biolegend 108741 Brilliant Violet 650TM anti-mouse CD45 Antibody 30-F11 Biolegend 103151 Brilliant Violet 711TM anti-mouse CD8a Antibody 53-6.7 Biolegend 100759 APC Rat IgG2a, κ Isotype Ctrl Antibody RTK2758 Biolegend 400512 PE/DazzleTM 594 Armenian Hamster IgG Isotype Ctrl Antibody HTK888 Biolegend 400951 PerCP/Cy5.5 Syrian Hamster IgG Isotype Ctrl Antibody SHG-1 Biolegend 402027 FITC Armenian Hamster IgG Isotype Ctrl Antibody HTK888 Biolegend 400906 PE Mouse IgG1, κ Isotype Ctrl (ICFC) Antibody MOPC-21 Biolegend 400140 PE/Cyanine7 Rat IgG2b, κ Isotype Ctrl Antibody RTK4530 Biolegend 400618 References Bernard, J.J., Cowing-Zitron, C., Nakatsuji, T., Muehleisen, B., Muto, J., Borkowski, A.W., Martinez, L., Greidinger, E.L., Yu, B.D., and Gallo, R.L. (2012).

    Generated:

    Article Title: YTHDF2 regulates self non-coding RNA metabolism to control inflammation and tumorigenesis
    Article Snippet: Antibodies for IF staining Reagents or Resource Source Identifier Dilution Alexa Fluor® 488 Donkey Anti-Guinea Pig IgG (H+L) Jackson ImmunoResearch 706-545-148 1/100 Alexa Fluor® 488 Donkey Anti-Mouse IgG (H+L) Jackson ImmunoResearch 715-545-150 1/100 Alexa Fluor® 594 Donkey Anti-Rabbit IgG (H+L) Jackson ImmunoResearch 711-585-152 1/100 Pan-cytokeratin Origene BP5069 1/200 RAB7 Abcam Ab50533 1/100 YTHDF2 (IHC and IF) Proteintech #247441-1-AP 1/500 YTHDF2 (Confocal) Abcam ab246514 1/200 dsRNA (IF) SCIONS 10010200 1/150 Cytokeratin 14 (IF) abcam Ab7800 1/1000 Cytokeratin 10 (IF) abcam Ab76318 1/5000 COX-2 (IF) Santa Cruz sc-376861 1/50 Supplementary Table S5. .. Antibodies for immunoblotting Reagents or Resource Source Identifier Dilution ATG5 Cell Signaling Technology 2630S 1/1000 ATG7 Cell Signaling Technology 8558S 1/1000 beta-actin Santa Cruz SC-47778 1/10000 COX-2 Santa Cruz SC-19999 1/500 FLAG-Tag (DYKDDDDK) Cell Signaling Technology 8146S 1/500 FLAG-Tag (DYKDDDDK) Cell Signaling Technology 14793S 1/500 FLAG-Tag (DYKDDDDK) Proteintech 20543-1-AP 1/2000 GAPDH Santa Cruz SC-47724 1/10000 HA-Tag Cell Signaling Technology 3724S 1/2000 HA-Tag Sigma H3663 1/2000 IGF2BP1 (IMP1) Cell Signaling Technology 8482S 1/1000 IGF2BP2 (IMP2) Cell Signaling Technology 14672S 1/1000 IGF2BP3 (IMP3) Cell Signaling Technology 57145S 1/1000 LC3B Cell Signaling Technology 3868S 1/500 METTL16 Cell Signaling Technology 17676S 1/2000 p62 Sigma P0067 1/2000 p62 Progen Biotechnik GmbH GP62-C 1/10000 TLR3 Cell Signaling Technology 6961S 1/500 TLR3 Abcam ab62566 1/500 TLR3 Abcam ab13915 1/500 YTHDC1 Abcam ab122340 1/1000 YTHDC2 Abcam ab176846 1/1000 YTHDF1 Proteintech 66745-1-Ig 1/2000 YTHDF2 Abcam ab220163 1/5000 YTHDF2 Proteintech 247441-1-AP 1/5000 YTHDF2 Cell Signaling Technology 80014S 1/2000 YTHDF3 Cell Signaling Technology 24206S 1/500 PARP1 Santa Cruz sc-8007 1/500 RAB5 Abcam ab18211 1/500 RAB7 Abcam ab50533 1/1000 YTHDF2 pS39 Ab Generated by ABclonal 1/1000 CNOT1 Proteintech 66507-1-lg 1/500 Supplementary Table S6. .. Antibodies for flow cytometric analysis Antibodies Clone Source Identifier FITC anti-mouse CD103 Antibody 2 E7 Biolegend 121420 FITC anti-mouse CD3 Antibody 17A2 Biolegend 100203 FOXP3 Monoclonal Antibody, PE NRRF-30 Thermo fisher 12-4771-82 PerCP/Cy5.5 anti-mouse/human CD45R/B220 Antibody RA3-6B2 Biolegend 103236 PerCP/Cyanine5.5 anti-mouse/human CD11b Antibody 101228 Biolegend 101228 PE/Cy7 anti-mouse CD279 (PD-1) Antibody 29F.1A12 Biolegend 109110 PE/Cyanine7 anti-mouse/human CD44 Antibody IM7 Biolegend 103030 PE/Cyanine7 anti-mouse Ly-6C Antibody HK1.4 Biolegend 128018 APC anti-mouse CD11C Antibody N418 Biolegend 117310 APC anti-mouse CD62L Antibody MEL-14 Biolegend 104412 Brilliant Violet 421TM anti-mouse I-A/I-E Antibody M5/114.15.2 Biolegend 107632 Brilliant Violet 421TM anti-mouse NK-1.1 Antibody PK136 Biolegend 100451 Brilliant Violet 510TM anti-mouse Ly-6G Antibody 1A8 Biolegend 127633 Brilliant Violet 605TM anti-mouse CD4 Antibody GK1.5 Biolegend 108741 Brilliant Violet 650TM anti-mouse CD45 Antibody 30-F11 Biolegend 103151 Brilliant Violet 711TM anti-mouse CD8a Antibody 53-6.7 Biolegend 100759 APC Rat IgG2a, κ Isotype Ctrl Antibody RTK2758 Biolegend 400512 PE/DazzleTM 594 Armenian Hamster IgG Isotype Ctrl Antibody HTK888 Biolegend 400951 PerCP/Cy5.5 Syrian Hamster IgG Isotype Ctrl Antibody SHG-1 Biolegend 402027 FITC Armenian Hamster IgG Isotype Ctrl Antibody HTK888 Biolegend 400906 PE Mouse IgG1, κ Isotype Ctrl (ICFC) Antibody MOPC-21 Biolegend 400140 PE/Cyanine7 Rat IgG2b, κ Isotype Ctrl Antibody RTK4530 Biolegend 400618 References Bernard, J.J., Cowing-Zitron, C., Nakatsuji, T., Muehleisen, B., Muto, J., Borkowski, A.W., Martinez, L., Greidinger, E.L., Yu, B.D., and Gallo, R.L. (2012).



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    Figure 5. The <t>m6A/YTHDF1/APC</t> pathway is required for axonal growth in cultured neurons and callosal neurons in vivo (A) Representative DIV6 neurons expressing shControl, shYthdf1, shMettl14, or shApc along with GFP. Scale bar, 50 μm. (B) Quantification of the longest axonal branch length (number of axons: shControl, n = 48; shYthdf1, n = 13; shMettl14, n = 16; shApc, n = 16) and the total number of axonal branch tips (shControl, n = 47; shYthdf1, n = 13; shMettl14; n = 16; shApc, n = 16). The results are normalized to the average of the shControl.
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    Figure 5. The m6A/YTHDF1/APC pathway is required for axonal growth in cultured neurons and callosal neurons in vivo (A) Representative DIV6 neurons expressing shControl, shYthdf1, shMettl14, or shApc along with GFP. Scale bar, 50 μm. (B) Quantification of the longest axonal branch length (number of axons: shControl, n = 48; shYthdf1, n = 13; shMettl14, n = 16; shApc, n = 16) and the total number of axonal branch tips (shControl, n = 47; shYthdf1, n = 13; shMettl14; n = 16; shApc, n = 16). The results are normalized to the average of the shControl.

    Journal: Cell reports

    Article Title: m 6 A RNA methylation-mediated control of global APC expression is required for local translation of β-actin and axon development.

    doi: 10.1016/j.celrep.2025.115727

    Figure Lengend Snippet: Figure 5. The m6A/YTHDF1/APC pathway is required for axonal growth in cultured neurons and callosal neurons in vivo (A) Representative DIV6 neurons expressing shControl, shYthdf1, shMettl14, or shApc along with GFP. Scale bar, 50 μm. (B) Quantification of the longest axonal branch length (number of axons: shControl, n = 48; shYthdf1, n = 13; shMettl14, n = 16; shApc, n = 16) and the total number of axonal branch tips (shControl, n = 47; shYthdf1, n = 13; shMettl14; n = 16; shApc, n = 16). The results are normalized to the average of the shControl.

    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-YTHDF1 Proteintech Cat#:17479-1-AP; RRID:AB_2217473 Anti -YTHDF2 Proteintech Cat#:24744-1-AP; RRID:AB_2687435 Anti-YTHDF3 Santa Cruz Biotech Cat#:Sc-377119; RRID:AB_2687436 Anti-METTL14 ATLAS ANTIBODIES (Sigma) Cat#:HPA038002; RRID:AB_10672401 Anti-Tubulin (Tyrosinated) Abcam Cat#:ab6160; RRID:AB_305328 Anti-APC Millipore Cat#:OP80; RRID:AB_2057371 Anti-APC-C (C-20) Santa Cruz Cat#:SC-896; RRID:AB_2057493 Anti-FMRP Abcam Cat#:ab17722; RRID:AB_2278530 Anti-HA-tag MBL Cat#:M180-3; RRID:AB_10951811 Anti-HA tag Abcam Cat#:ab9110; RRID:AB_307019 Anti-GAPDH (14C10) Cell Signaling Cat#:2118; RRID:AB_561053 Anti-Puromycin Millipore Cat#:MABE343; RRID:AB_2566826 Anti-DCX Abcam Cat#:ab18723; RRID:AB_732011 Anti-CRMP2 Biolegend Cat#:869001; RRID:AB_732011 Anti-GFP Abcam Cat#:ab13970; RRID:AB_300798 Anti-Tau Abcam Cat#:ab75714; RRID:AB_1310734 Anti-MAP2 Abcam Cat#:ab5392; RRID:AB_2138153 Anti-Beta-actin Cayman Chemicals Cat#:32127; RRID:AB_2810170 Anti-Cre Recombinase Millipore Cat#:MAB3120; RRID:AB_2085748 Anti-Tubb2b Abcepta Cat#:AP11940a; RRID:AB_10820514 Goat Anti-Mouse IgG (H + L) cross-adsorbed secondary antibody Alexa Fluor 488 Thermofisher Cat#:A11001; RRID:AB_2534069 Goat Anti-Mouse IgG (H + L) cross-adsorbed secondary antibody Alexa Fluor 568 Thermofisher Cat#:A11004; RRID:AB_2534072 Goat Anti-Mouse IgG (H + L) cross-adsorbed secondary antibody Alexa Fluor 633 Thermofisher Cat#:A21050; RRID:AB_2535718 Goat Anti-Rabbit IgG (H + L) cross-adsorbed secondary antibody Alexa Fluor 633 Thermofisher Cat#:A21070; RRID:AB_2535731 Donkey anti-Chicken IgY (H + L) Highly Cross Adsorbed Secondary Antibody, Alexa Fluo 568 Thermofisher Cat# A78950; RRID:AB_2921072 Bacterial and virus strains NEB Stable Competent E. coli New England Biolabs Cat#:C3040H Chemicals, peptides, and recombinant proteins Puromycin InvivoGen Cat#:58-58-2 ProLong Diamond antifade mountant Thermofisher Cat#:P36965 DPBS Thermofisher Cat#:10010023 Neurobasal medium Thermofisher Cat#:10888022 MEM Thermofisher Cat#:11095080 Penicillin/Streptomycin Thermofisher Cat#:15140122 (Continued on next page) 18 Cell Reports 44, 115727, June 24, 2025

    Techniques: Cell Culture, In Vivo, Expressing

    Figure 7. Model depicting the regulation of axon development via the m6A/YTHDF1/ APC pathway, which governs β-actin mRNA transport and local translation

    Journal: Cell reports

    Article Title: m 6 A RNA methylation-mediated control of global APC expression is required for local translation of β-actin and axon development.

    doi: 10.1016/j.celrep.2025.115727

    Figure Lengend Snippet: Figure 7. Model depicting the regulation of axon development via the m6A/YTHDF1/ APC pathway, which governs β-actin mRNA transport and local translation

    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-YTHDF1 Proteintech Cat#:17479-1-AP; RRID:AB_2217473 Anti -YTHDF2 Proteintech Cat#:24744-1-AP; RRID:AB_2687435 Anti-YTHDF3 Santa Cruz Biotech Cat#:Sc-377119; RRID:AB_2687436 Anti-METTL14 ATLAS ANTIBODIES (Sigma) Cat#:HPA038002; RRID:AB_10672401 Anti-Tubulin (Tyrosinated) Abcam Cat#:ab6160; RRID:AB_305328 Anti-APC Millipore Cat#:OP80; RRID:AB_2057371 Anti-APC-C (C-20) Santa Cruz Cat#:SC-896; RRID:AB_2057493 Anti-FMRP Abcam Cat#:ab17722; RRID:AB_2278530 Anti-HA-tag MBL Cat#:M180-3; RRID:AB_10951811 Anti-HA tag Abcam Cat#:ab9110; RRID:AB_307019 Anti-GAPDH (14C10) Cell Signaling Cat#:2118; RRID:AB_561053 Anti-Puromycin Millipore Cat#:MABE343; RRID:AB_2566826 Anti-DCX Abcam Cat#:ab18723; RRID:AB_732011 Anti-CRMP2 Biolegend Cat#:869001; RRID:AB_732011 Anti-GFP Abcam Cat#:ab13970; RRID:AB_300798 Anti-Tau Abcam Cat#:ab75714; RRID:AB_1310734 Anti-MAP2 Abcam Cat#:ab5392; RRID:AB_2138153 Anti-Beta-actin Cayman Chemicals Cat#:32127; RRID:AB_2810170 Anti-Cre Recombinase Millipore Cat#:MAB3120; RRID:AB_2085748 Anti-Tubb2b Abcepta Cat#:AP11940a; RRID:AB_10820514 Goat Anti-Mouse IgG (H + L) cross-adsorbed secondary antibody Alexa Fluor 488 Thermofisher Cat#:A11001; RRID:AB_2534069 Goat Anti-Mouse IgG (H + L) cross-adsorbed secondary antibody Alexa Fluor 568 Thermofisher Cat#:A11004; RRID:AB_2534072 Goat Anti-Mouse IgG (H + L) cross-adsorbed secondary antibody Alexa Fluor 633 Thermofisher Cat#:A21050; RRID:AB_2535718 Goat Anti-Rabbit IgG (H + L) cross-adsorbed secondary antibody Alexa Fluor 633 Thermofisher Cat#:A21070; RRID:AB_2535731 Donkey anti-Chicken IgY (H + L) Highly Cross Adsorbed Secondary Antibody, Alexa Fluo 568 Thermofisher Cat# A78950; RRID:AB_2921072 Bacterial and virus strains NEB Stable Competent E. coli New England Biolabs Cat#:C3040H Chemicals, peptides, and recombinant proteins Puromycin InvivoGen Cat#:58-58-2 ProLong Diamond antifade mountant Thermofisher Cat#:P36965 DPBS Thermofisher Cat#:10010023 Neurobasal medium Thermofisher Cat#:10888022 MEM Thermofisher Cat#:11095080 Penicillin/Streptomycin Thermofisher Cat#:15140122 (Continued on next page) 18 Cell Reports 44, 115727, June 24, 2025

    Techniques: